Blotting Buffers & Membranes GEL BLOTTING PAPER - FN100 Gel blotting paper with an extremely smooth surface and a 0.35mm thickness. Made from the purest naturally occurring raw materials, offering the maximum degree of absorption and cellulose content. Fast running and high absorption capacity; ideal ombination of chromatography and gel blotting paper. The ideal general purpose blotting paper for Southern, Northern and Western blotting, gel lifting, sequencing, buffer wicking and semi-dry blotting.
| Superior uniformity across the entire contact area in the blotting transfer system | | Absorption and improvement in the transport f transfer buffer after capillary and semi-dry blotting | | Absorption and improvement in the transport f transfer buffer after capillary and semi-dry blotting | | To prevent direct contact between the blotting membrane and porous cover plate of the vacuum chamber for dot/slot blotting of RNA and DNA |
Towbin Buffer, 10x, for Western BlottingSupplied as a 10x concentrate: (0.25M Tris and 1.92M Glycine in aqueous solution. Working buffer: dilute 100ml of 10x concentrate with 200ml of methanol and 700ml distilled water.Semi-Dry Blotting Buffer KitSupplied as a ready-to-use kit, comprising of 3 components for Western blotting in the semi-dry blotting units.
Buffer 1. (Conc. Anode buffer): 0.3M Tris and 20% Methanol in aqueous solution.
Buffer 2. (Diluted Anode buffer): 0.03M Tris and 20% Methanol in aqueous solution.
Buffer 3. (Cathode buffer): 0.25M Tris/HCl (pH 9.4), 0.04M 6-AminocaprBLOTTING MEMBRANESScie-Plas offers 5 different types of blotting membrane for use with all transfer techniques. Each membrane is available in 10 x 10cm and 20 x 20cm formats and in 0.2 and 0.45µ
m pore sizes for the binding of low (<20kDa) and high (>20kDa) molecular weight proteins and nucleic acids.Nitrocellulose Blotting MembranesNitrocellulose membranes are the most popular membranes for Western Southern and Northern blotting. The membranes bind both proteins and nucleic acids. Nitrocellulose exhibits high binding capacity and has low background.| 0.02µm pore size, 100mm x 100mm | EB-MEM-NC20 | 25 Sheets | 165,00 € |  |
| 0.02µm pore size, 200mm x 200mm | EB-MEM-NC20L | 25 Sheets | 460,00 € |  |
| 0.45µm pore size, 100mm x 100mm | EB-MEM-NC45 | 25 Sheets | 165,00 € | |
| 0.45µm pore size, 200mm x 200mm | EB-MEM-NC45L | 25 Sheets | 460,00 € | |
Supported Nitrocellulose Blotting MembranesAs per nitrocellulose but more robust for frequent handling.| 0.02µm pore size, 100mm x 100mm | EB-MEM-SNC20 | 25 Sheets | 230,00 € | |
| 0.45µm pore size, 100mm x 100mm | EB-MEM-SNC45 | 25 Sheets | 230,00 € | |
Polyvinylidene Fluoride (PVDF) Blotting MembranesA hydrophobic membrane with much higher protein-binding capacity than nitrocellulose.| 0.02µm pore size, 100mm x 100mm | EB-MEM-PVDF20 | 25 Sheets | 230,00 € | |
| 0.02µm pore size, 200mm x 200mm | EB-MEM-PVDF20L | 25 Sheets | 620,00 € | |
| 0.45µm pore size, 100mm x 100mm | EB-MEM-PVDF45 | 25 Sheets | 230,00 € | |
| 0.45µm pore size, 200mm x 200mm | EB-MEM-PVDF45L | 25 Sheets | 620,00 € | |
Supported Nylon-66 Blotting MembranesA more versatile membrane, with reduced background to increase sensitivity, for the binding of nucleic acids after semi-dry blotting, capillary Southern and Northern transfer; more robust for frequent handling.| 0.02µm pore size, 100mm x 100mm | EB-MEM-SN20 | 25 Sheets | 230,00 € | |
| 0.02µm pore size, 200mm x 200mm | EB-MEM-SN20L | 25 Sheets | 620,00 € | |
| 0.45µm pore size, 100mm x 100mm | EB-MEM-SN45 | 25 Sheets | 230,00 € | |
| 0.45µm pore size, 200mm x 200mm | EB-MEM-SN45L | 25 Sheets | 620,00 € | |
Positively Charged Supported Nylon-66 Blotting MembranesHigher binding affinity than supported Nylon-66 for negatively charged molecules such as nucleic acids during Southern and Northern transfer; more robust for frequent handling.| 0.02µm pore size, 100mm x 100mm | EB-MEM-SN20 | 25 Sheets | 230,00 € | |
| 0.02µm pore size, 200mm x 200mm | EB-MEM-SN20L | 25 Sheets | 620,00 € | |
| 0.45µm pore size, 100mm x 100mm | EB-MEM-SN45 | 25 Sheets | 230,00 € | |
| 0.45µm pore size, 200mm x 200mm | EB-MEM-SN45L | 25 Sheets | 620,00 € | |
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