Dot and Slot Blot Microfiltration Manifolds


Available in alphanumeric 48 and 96 sample throughputs, Scie-Plas's dot and slot blot microfiltration manifolds provide an easy and reproducible means to hybridise proteins and nucleic acids in solution onto membranes. These manifolds, which are machined from high-density acrylic, have precision-lapped mating surfaces that ensure uniform filter/membrane contact without gaskets and 'O' rings. Manifold assembly is simple, being achieved by the alignment and tightening of stainless steel thumbscrews and mating screws, while filters can be cut down to the exact size of the template. Standard manifold configurations are available with the DHM-48 and -96 units providing 48 and 96 dots, 3mm in diameter, that are compatible with the configuration of 96-well microtitre plates. The SHM-48 units focus up to 48 samples into thin lines, less than 0.5 x 6mm in size, making densitometric quantitation more reproducible. Each hybridisation manifold requires a vacuum pump or water aspirator equivalent to 600mm Hg (0.8 Bar).




Benefits
Rapid transfer assured within minutes dot/slot microfiltration manifold allows unfractionated samples to be immobilised onto a membrane for immediate screening with nucleic acid probes or antibodies
Available in 3 different formats:DHM-48 - for 5 x 12.8cm membranes - 48-sample throughput

DHM-96 - for 7.5 x 11.3cm membranes - 96-sample throughput

SHM-48 - for 5 x 12.8cm membranes - 48-sample throughput
Compatibility (DHM Only) with multichannel pipettes and standard 96-well microtitre plates ensures fast loading of the manifold
Simple design based on the alignment of stainless steel thumbscrews and mating screws - guarantees fast and easy set up without the need for gaskets and 'O' rings
Dot/slot blot imaging and quantitation available on the Vision 11 system


Ordering Information

Complete System

48-well Dot Blot Hybridisation Manifold in 3 x 16 array, comprising paired upper dot-forming modules for dots 3mm in diameter, and lower vacuum chamber module.DHM-48695,00 €
96-well Dot Blot Hybridisation Manifold in 8 x 12 array, comprising paired upper dot-forming modules for dots 3mm in diameter, and lower vacuum chamber module.DHM-96730,00 €Add to your cart
48-well Slot Blot Hybridisation Manifold in 3 x 16 array, comprising paired upper slot-forming module for slots 0.5 x 6mm in size, and lower vacuum chamber module.SHM-48895,00 €

Technical Specification

SHM-48

see ordering details
Unit Dimensions (WxDxH)18.5x7.5x7cm
Maximum Sample Capacity48
Dot Diameter -
Slot Dimensions (WxL)3x12mm
Sample Volume / Dot -
Sample Volume / Slot300°l
Minimum Membrane Size (WxL)5x12.8cm
Vacuum Port External Diameter7mm
Vacuum Port Internal Diameter6mm
Maximum Operating Temperature65°C
Working Pressure of Pump or Water Aspirator600mm Hg (0.8 Bar)

DHM-48

see ordering details
Unit Dimensions (WxDxH)18.5x7.5x7cm
Maximum Sample Capacity48
Dot Diameter3mm
Slot Dimensions (WxL) -
Sample Volume / Dot100°l
Sample Volume / Slot -
Minimum Membrane Size (WxL)5 x 12.8cm
Vacuum Port External Diameter7mm
Vacuum Port Internal Diameter6mm
Maximum Operating Temperature65°C
Working Pressure of Pump or Water Aspirator600mm Hg (0.8 Bar)

DHM-96

see ordering details
Unit Dimensions (WxDxH)17x10.5x7cm
Maximum Sample Capacity96
Dot Diameter3mm
Slot Dimensions (WxL) -
Sample Volume / Dot100°l
Sample Volume / Slot -
Minimum Membrane Size (WxL)7.5x11.3cm
Vacuum Port External Diameter7mm
Vacuum Port Internal Diameter6mm
Maximum Operating Temperature65°C
Working Pressure of Pump or Water Aspirator600mm Hg (0.8 Bar)
Benefits
Ordering Information
Technical Specifications


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