The V10-SDB and V20-SDB Semi-Dry Blotters


Semi-dry blotting with Scie-Plas V10-SDB and V20-SDB units

The V10-SDB 10 x 10cm and V20-SDB 20 x 20cm semi-dry blotters are ideal for fast transfer of proteins and nucleic acids without the need for costly accessories such as gel cassettes and tanks. Corrosion-free platinum-coated titanium and stainless steel plate electrodes, corresponding to the anode and cathode, maximise the active area of transfer so that full electrical contact is made between the gel and the membrane, allowing transfer to be completed within an hour with the minimum of heat dissipation, buffer consumption and mess in the laboratory, normally associated with wet blotting techniques.

Three sheets of blotting paper, saturated with transfer buffer, are first superimposed over the anode plate, followed by the membrane, the gel and a further 3 sheets of blotting paper. The cathode plate is then placed over the blotting sandwich and the current applied at 0.8mAcm-2 for 1 to 2 hours.



Benefits
2 sizes available:V10-SDB - 10 x 10cm format - accommodates one 10 x 10cm mini-gel

V20-SDB - 20 x 20cm format - accommodates up to four 10 x 10cm mini-gels or one 20 x 20cm maxi-gel
Gels can be stackedfor higher throughput blotting
Rapid transfer assured within 1 hourfor lower molecular weight proteins and nucleic acids; larger molecules transferred in 2 hours
Semi-dry formatminimises buffer consumption, with no need for mess and additional accessories
Colour-coded, corrosion-free plate electrodesplatinum-coated titanium anode and stainless steel cathode maximise the transfer area, so that full electrical contact is made between the gel and the membrane, while corrosionresistant metal prolongs lifespan and durability


Ordering Information

Complete System

10 x 10cm semi-dry blotterV10-SDB925,00 €
20 x 20cm semi-dry blotterV20-SDB1.850,00 €Add to your cart

Technical Specification

V10-SDB

see ordering details
Unit Dimensions (WxDxH)17x7.5x17cm
Active Transfer Area (WxL)10x10cm
Sample ThroughputOne 10 x 10cm mini-gel
Recommended Current Setting80mA / 0.8mAcm-2
Power Output Connectors (diameter)4mm
Recommended Power SuppliesEV243

V20-SDB

see ordering details
Unit Dimensions (WxDxH)27x7.5x27cm
Active Transfer Area (WxL)20x20cm
Sample ThroughputFour 10 x 10cm mini-gels;
One 20 x 20cm maxi-gel
Recommended Current Setting320mA / 0.8mAcm-2
Power Output Connectors (diameter)4mm
Recommended Power SuppliesEV265, 261 & 215
Benefits
Ordering Information
Technical Specifications


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