The TV400-DGGE Denaturing Gradient Gel Electrophoresis System
The TV400-DGGE denaturing Gradient Gel Electrophoresis system is a cost-effective solution for researchers studying mutations and DNA polymorphisms critical in disease aetiology and genetic diversity. Designed primarily for parallel denaturing Gradient Gel Electrophoresis (DGGE), where electrophoresis and the denaturing gradient run in the same direction, the TV400-DGGE has a maximum 96-sample throughput compatible with standard microplates and thermal cycler blocks. A 100ml gradient mixer is also included to pour gradient gels using our newly designed gel-running module and cam-caster, while a 400W heater, regulated by an external temperature control unit connected to a heat sensor within the gel tank, allows the gel temperature to be set to the predetermined melting temperature (Tm) of the PCR®-amplified DNA polymorphism or mutation of interest. 400W heating element - manually controlled by a temperature control unit connected to a PT100 heat sensor - enables the gel and buffer temperature to be set in 1°C increments to a predetermined Tm, to a maximum 70°
| Maximum 96-sample throughput | using two 48-sample combs - allows samples to be transferred quickly and easily to the each gel from standard 96-well microplates or thermal cycler blocks | | Large format | 20.5 x 20cm glass plates allow gradient gels to be poured containing wider ranges of denaturant concentration, maximising sensitivity and resolution | | 100ml gradient mixer | used with the new-style TV400-GRM and casting base to make two 1mm parallel denaturing gradient gels | | Formamide, urea and acrylamide available | to pour denaturing gradient gels - contact info@alphametrix.de | | Can also be adapted for constant denaturing gel electrophoresis (CDGE) | if the denaturant concentration required for partial melting of the DANN target sequence is already known |
Complete System| Twin-plate maxi-gel unit with GRM, heated gel tank with PT100 temperature sensor and thermometer, lid, 2 x (20.5 x 20cm; W x H) plain glass plates, 2 x (20.5 x 20cm) notched glass plates, 4 x 1mm spacers, 2 x spacer aligners and 2 x 1mm thick 24-sample combs, casting base, 2 x silicone seals and 1 x dummy plate; plus GM100 gradient mixer and heat sensor control unit | TV400-DGGE | please contact us |  |
Replacement Parts & Accessories| 1 x TV400 gel-running module | TV400-GRM | 895,00 € |  |
| 2 x (20.5 x 20cm) plain glass plates for TV400 | TV400-PG | 60,00 € |  |
| 2 x (20.5 x 20cm) notched glass plates for TV400 | TV400-NG | 85,00 € | |
| 1 x dummy plate | TV400-DP | 75,00 € | |
| 2 x 1mm thick spacers for TV400 | TV400-S1 | 30,00 € | |
| 2 x spacer aligners for TV400 | TV400-SA | 59,00 € | |
| 1 x cam-pin casting base with 2 silicone seals | TV400-CB | 315,00 € | |
| 1 x caster system for 2 x (20.5 x 20cm) gradient maxi-gels | TV400-MC2 | 890,00 € | |
| 2 x 0.2mm thick, platinum electrode wire | PT-0.2 | 128,00 € | |
| 2 x 1 metre power leads with shrouded 4mm power output connectors | CABLE-4 | please contact us | |
Reagents & Chemicals
| Unit Dimensions (WxDxH) | 28.5x15x29cm | | Inner Tank Dimensions (WxDxH) | 27x12.5x26cm | | Plate Dimensions (WxHxT) | 20.5x20x0.4cm | | Standard Spacer Dimensions (WxHxT) | 2x20x0.1cm | | Active Gel Dimensions (W x H) | 16.5 x 17.5cm | | Sample Capacity | 2 x 24 | | Recommended Buffer Volume | | Inner Buffer Chamber | 700ml | | Gel Tank | 4200ml | | Recommended Running Conditions for Denaturing/Native PAGE Gel | | Voltage | 175 - 225V
(8.75 - 11.25V/cm) | | Current | 20 - 30mA | | Time | 2.5 - 3.5h | | Temperature Range Thermometer | 0 - 100°C | | Power Output of Heating Element | 400W | | Heating Element Dimensions (L x D) | 14 x 1.5cm | | Operating Range of PT100 Temperature Sensor | -50 to +200°C | | PT100 Temperature Sensor Dimensions (L x D) | 14 x 0.3cm | | Casting Base Silicone Seal Dimensions (WxLxH) | 1.7x22.5x0.8cm | | Power Output Connectors (diameter) | Shrouded, 4mm | | Recommended Power Supplies | Consort EV243 | | HS-UNIT | | Unit Dimensions (WxDxH) | 9.6x14x8cm | | Recommended Temperature Control Range | 50 to 70°C | | Temperature Control Accuracy | +/-1°C |
| Order-Nr | Thickness(mm) | Sample Throughput | Tooth Widt (mm) | Tooth Height (mm) | Max. Spacing (mm) | Sample Volume
in a 5mm
Deep Weel (µl) | Price | Add | | TV400-C1-24 (standard) | 1 | 24 | 4.75 | 20 | 2 | 95 | please contact us | | | TV400-C1-48 (optional) | 1 | 48 | 2.35 | 20 | 1 | 47 | please contact us | | | ORDERNR-MC = Multichannel pipette compatible |
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